Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: API-20 E biochemical profile of the Aeromonas isolates.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques:

Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: Incidence (%) of Aeromonas spp. isolated from fish and human samples.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques: Isolation, Infection, Muscles

Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: ( A ) Representation of polymerase chain reaction (PCR) positives for 16S rRNA of the genus Aeromonas with an amplicon size of 953 bp. L: 100 bp ladder; lanes 1–5, 9–11, and 13–15: positive samples; lanes 6–8 and 12: negative samples; lane Pos: positive control. ( B ) Representation of polymerase chain reaction (PCR) positives for 16S rRNA of genus Aeromonas hydrophila with an amplicon size of 625 bp. L: 100 bp ladder; lanes 1, 4, 13, and 14: positive samples; lanes 2, 3, 5, 9, 10, 11, and 15: negative samples; lane pos: positive control; lane neg: negative control. ( C ) Representation of polymerase chain reaction (PCR) positives for 16S rRNA of aer and hly gene amplification of Aeromonas hydrophila isolates. L: 100 bp ladder; for the aer gene lanes (13 and 14): positive samples. Lane (1 and 4): negative sample. Lane Pos: positive control and lane Neg: negative control. For the hly the, lanes 1, 4, 13, and 14: negative samples. Lane Pos: positive control and lane Neg: negative control.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques: Polymerase Chain Reaction, Amplification, Positive Control, Negative Control

Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: Antibiotic suceptibilty of molecularly identified Aeromonas spp. against 16 antibiotics.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques:

Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: Distribution of antibiotic resistance (%) of Aeromonas spp. isolates and MAR index.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques: Muscles

Journal: Biology

Article Title: Antibiotic Resistance, Virulence Gene Detection, and Biofilm Formation in Aeromonas spp. Isolated from Fish and Humans in Egypt

doi: 10.3390/biology12030421

Figure Lengend Snippet: Biofilm production by Aeromonas spp. using microtiter plate method.

Article Snippet: Twenty-five g of the collected samples, including fish tissues and organs from both tilapia and mugil and human skin swabs and fecal samples were enriched with alkaline peptone water (Merck, Germany) and incubated under shaking conditions at 37 °C for 24 h. After overnight incubation, serial dilutions were made, then 100 μL aliquots from these dilutions were streaked onto selective Aeromonas agar media (Himedia, Egypt), and the plates were incubated at 37 °C for 24 h under aerobic conditions.

Techniques: Control, Muscles